Anti-GRP78 BiP Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
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HSPA5
Host
Storage
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Store at 2-8°C for up to 2 weeks. store at -20°C for long term storage in small aliquots. Avoid freeze/thaw cycles.
Clonality
Clone Number
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Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/1000 IHC: 1/100-1/500 IF: 1/10-1/50
Immunogen
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This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the Central region of human HSPA5.
Molecular Weight
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Calculated MW:73 kDa; Observed MW: 73 kDa
Formulation
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Liquid in PBS containing 0.09% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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3309
Uniprot ID
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P11021
Background
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Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen . Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) . In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation.
Research Area
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Tags & Cell Markers
Synonyms
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78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78