Anti-GRP78 BiP (9E4) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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HSPA5
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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9E4-2A7-H6
Isotype
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IgG1
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IF: 1/50-1/200
Immunogen
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Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
Molecular Weight
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Calculated MW: 72 kDa; Observed MW: 78 kDa
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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3309
Uniprot ID
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P11021
Background
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When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Research Area
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Tags & Cell Markers
Synonyms
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HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP