Anti-GEN1 Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
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GEN1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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R09-1B8
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000
Immunogen
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A synthetic peptide of human GEN1
Molecular Weight
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Calculated MW: 103 kDa; Observed MW: 103 kDa
Formulation
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50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Form
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Liquid
Gene ID
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348654
Uniprot ID
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Q17RS7
Background
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Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (PubMed:19020614, PubMed:26682650). Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction (PubMed:26578604). Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA (PubMed:28049850). Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates (PubMed:26578604).
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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Gen