Anti-GCSm-γ Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
-
GCLM
Host
Storage
-
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clonality
Clone Number
-
-
Isotype
-
IgG
Reactivity
Application
Application Details
-
WB: 1/500-1/1000 IHC: 1/50-1/200
Immunogen
-
Synthetic peptide corresponding to Human GCSm-γ.
Molecular Weight
-
Calculated MW: 30kDa
Formulation
-
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Form
-
Liquid
Gene ID
-
2730
Uniprot ID
-
P48507
Background
-
Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-γ-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines. The human γ-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
Research Area
-
-
Synonyms
-
Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit; GLCLR; GCLM