Anti-EIF3E Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
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EIF3E
Host
Storage
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Store at 2-8°C for up to 2 weeks. store at -20°C for long term storage in small aliquots. Avoid freeze/thaw cycles.
Clonality
Clone Number
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-
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/1000
Immunogen
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This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the Central region of human EIF3E.
Molecular Weight
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Calculated MW:52 kDa; Observed MW: 40-55 kDa
Formulation
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Liquid in PBS containing 0.09% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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3646
Uniprot ID
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P60228
Background
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Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression . Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway . May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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Eukaryotic translation initiation factor 3 subunit E, eIF3e, Eukaryotic translation initiation factor 3 subunit 6 , Viral integration site protein INT-6 homolog, eIF-3 p48, EIF3E