Anti-DNA PKcs (6D1) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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PRKDC
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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6D1-C11-F10
Isotype
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IgG1
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Immunogen
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Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
Molecular Weight
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Calculated MW: 469 kDa; Observed MW: 450 kDa
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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5591
Uniprot ID
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P78527
Background
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The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.
Research Area
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Epigenetics and Nuclear Signaling
Synonyms
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PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460