Anti-Crotonyl-Histone H2B (Lys12) Rabbit Monoclonal Antibody
Product Information
Downloads & Supports
Target
-
H2BC4
Host
Storage
-
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
-
R03-1J-4
Isotype
-
IgG
Reactivity
Application
Application Details
-
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Immunogen
-
Peptide
Molecular Weight
-
Calculated MW:14 kDa;Observed MW: 14 kDa
Formulation
-
Liquid in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Form
-
Liquid
Gene ID
-
3018
Uniprot ID
-
P33778
Background
-
Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Crotonylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine crotonylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine crotonylation suggest that it is mechanistically and functionally different from histone lysine acetylation. Specifically, in both human somatic and mouse male germ cell genomes, histone crotonylation marks either active promoters or potential enhancers. Crotonylation of histone H2B at Lys11 may play a vital role in the epigenetic modulation, including chromatin remodeling and DNA transcriptional regulation.
Research Area
-
Epigenetics and Nuclear Signaling
Synonyms
-
H2BK11cr, Histone H2B.1 A；Histone H2B.a (H2B/a)；Histone H2B.g (H2B/g)；Histone H2B.h (H2B/h)；Histone H2B.k (H2B/k)；Histone H2B.l (H2B/l)