Anti-CPSF4 Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
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CPSF4
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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-
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000
Immunogen
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Fusion protein of human CPSF4
Molecular Weight
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Calculated MW: 30 kDa; Observed MW: 30 kDa
Formulation
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pH 7.4 PBS, 0.05% NaN3, 40% Glycerol
Form
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Liquid
Gene ID
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10898
Uniprot ID
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O95639
Background
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Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Research Area
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Microbiology
Synonyms
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NAR; NEB1; CPSF30