Anti-BRaf(V600E) Mouse Monoclonal Antibody

Anti-BRaf(V600E) Mouse Monoclonal Antibody
Anti-BRaf(V600E) Mouse Monoclonal Antibody

Anti-BRaf(V600E) Mouse Monoclonal Antibody

Mouse IgG2b Monoclonal for Braf detection, Test with WB, IHC-P, IP, ELISA in Human, Mouse, Rat

Reactivity
  • Human
  • Mouse
  • Rat
Application
  • WB
  • IHC-P
  • IP
  • ELISA
Host
  • Mouse
Catalog Number
    ABXB07271
$680.00

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United States

Product Information

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Target
    Braf
Host
  • Mouse
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Monoclonal
Clone Number
    3A5-A6-A8
Isotype
    IgG2b
Reactivity
  • Human
  • Mouse
  • Rat
Application
  • WB
  • IHC-P
  • IP
  • ELISA
Application Details
    WB: 1/100-1/1000 IHC-P：1/100-1/200 ELISA: 1/100-1/5000
Immunogen
    A synthetic peptide from the internal region of BRaf(V600E), human origin.
Molecular Weight
    -
Formulation
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Form
    Liquid
Gene ID
    109880
Uniprot ID
    P28028
Background
    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2 (By similarity).
Research Area
    Immunology
Synonyms
    Serine/threonine-protein kinase B-raf,Proto-oncogene B-Raf
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Product Image

Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal