Anti-BRAF (4E1) Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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BRAF
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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4E1-E1-F9
Isotype
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IgG2a
Reactivity
Application
Application Details
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WB: 1/500-1/1000
Immunogen
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Purified recombinant human B Raf protein fragments expressed in E.coli.
Molecular Weight
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Calculated MW: 84 kDa; Observed MW: 87 kDa
Formulation
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Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
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Liquid
Gene ID
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673
Uniprot ID
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P15056
Background
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Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.
Research Area
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Cell Biology
Synonyms
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BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1