Anti-Arfaptin 1 Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
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ARFIP1
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clonality
Clone Number
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-
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/200 IF: 1/50-1/200
Immunogen
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Synthetic peptide corresponding to Human Arfaptin 1.
Molecular Weight
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Calculated MW: 42kDa
Formulation
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1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Form
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Liquid
Gene ID
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27236
Uniprot ID
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P53367
Background
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Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.
Research Area
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Synonyms
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Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1; Arfaptin1