Anti-AQP0 Rabbit Polyclonal Antibody
Product Information
Downloads & Supports
Target
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MIP
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clonality
Clone Number
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-
Isotype
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IgG
Reactivity
Application
Application Details
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WB: 1/500-1/1000 IHC: 1/50-1/200
Immunogen
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Synthetic peptide corresponding to Human AQP0.
Molecular Weight
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Calculated MW: 28kDa
Formulation
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1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Form
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Liquid
Gene ID
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4284
Uniprot ID
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P30301
Background
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AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.
Research Area
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Synonyms
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Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26; MIP; AQP0; Aquaporin 0; Aquaporin0;