The ZIC1/2/3 Colorimetric Cell-Based ELISA provides a lysate-free, convenient, and high-throughput approach for sensitive detection of ZIC1/2/3 protein expression profile in cells. The kit can be used for measuring the relative amounts of ZIC1/2/3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on ZIC1/2/3.