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Mouse IgG1 Monoclonal for eGFP detection, Test with WB in Species-independent

Reactivity
  • Species-independent
Application
  • WB
Host
  • Mouse
Catalog Number
    TGXB00018
$220.00

Shipping To:
United States

Product Information

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Target
    eGFP
Host
  • Mouse
Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
  • Monoclonal
Clone Number
    3F11-B12-E7-F12
Isotype
    IgG1
Reactivity
  • Species-independent
Application
  • WB
Application Details
    WB: 1/1000-1/10000
Immunogen
    Purified recombinant EYFP.tag full length expressed in E.coli.
Molecular Weight
    Calculated MW: 27 kDa; Observed MW: Refer to figures
Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Form
    Liquid
Gene ID
    -
Uniprot ID
    C8CHS1
Background
    Swiss-Prot Acc.C8CHS1.Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization.
Research Area
    Tags & Cell Markers
Synonyms
    GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein.
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Product Image

Western blot analysis of EGFP/EYFP in Rosetta E.coli induced by 0(A)or 0.1mM (B)IPTG using EGFP/EYFP antibody.
Western blot analysis of EGFP/EYFP in CHO-K1 transfected by different EGFPfusion proteins or nontransfected (A) cell lysates using EGFP/EYFP antibody.