Anti-CD33 Mouse Monoclonal Antibody
Product Information
Downloads & Supports
Target
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CD33
Host
Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Clonality
Clone Number
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2A2-W5-J8
Isotype
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IgG2a/Kappa
Reactivity
Application
Application Details
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WB：1/500-1/1000
Immunogen
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Peptide
Molecular Weight
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Calculated MW: 67 kDa; Observed MW:67 kDa
Formulation
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Liquid in PBS, Glycerol and BSA
Form
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Liquid
Gene ID
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945
Uniprot ID
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P20138
Background
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Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
Research Area
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Tumor biology
Synonyms
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Siglec-3