Product Information
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Amine Magnetic Beads is magnetic bead with surface functional group -NH2. The magnetic beads consist of Fe3O4 magnetic sphere core and being coated with dextran. Through chemical modification of dextran, the primary amino group (-NH2) are joined to the magnetic beads through a short hydrophilic linker. The hydrophilic surface ensures the magnetic beads excellent dispersion ability and easy handling property in a wide variety of buffers.
The magnetic beads with surface-reactive amino groups allow immobilization of ligands such as proteins, peptides, carbohydrates or other target specific molecules.
Specifications
| Core | Iron Oxide (Fe3O4) |
| Layer | Dextran |
| Functional group | primary amino group (-NH2) |
| Mean Diameter of Particles | ~1 um |
| Storage Buffer | PBS pH-7.4 with 0.09% Sodium Azide and 0.02% Tween-20 |
| Storage temp. | 2 ~ 8 ℃ |
| Ligand density | 0.2 mM |
Additional material required
-
• MES Buffer (pH 6.0):
100 mM MES and 500 mM NaCl
• PBS, pH 7.4:
137 mM NaCl, 8.1 mM Na2HPO4,
1.47 mM KH2PO4 and 2.7 mM KCl
• Quench Buffer:
TBS,
pH 8.0 or 5-10 mM hydroxylamine
• Desired antibody or ligand
• Magntic stand: Magdorf for the best performance
• EDC [1-(3-dimethylaminopropyl)-3-ethylcarbo- diimide hydrochloride], C8H17N3·HCl, MW = 191.7, CAS No. 25952-53-8
• MES [2-(morpholino) ethanesulfonic acid], C6H13NO4S·H2O, MW = 213.25, CAS No.145224-94-8
• NHS [N-hydroxysuccinimide], C4H5NO3, MW = 115.09, CAS No. 6066- 82-6
• Tilt rotation device or vortexer
• Eppendorf tubes & pipet