4% Paraformaldehyde (PFA) Solution In PBS (With DEPC)
Product Information
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Catalog Number
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ARXA00001
Specifications
| Product Name | 4% Paraformaldehyde (PFA) Solution in PBS (with DEPC) |
|---|---|
| Catalog Number | ARXA00001 |
| Form | Liquid |
| Physical State | The solution should be clear, colorless, with no precipitate. |
| Size | 500mL |
| Application | Preserving Samples, Fixing Cells For Histology |
| Storage | Store at -20˚C for one year. |
| CAS Number | 30525-89-4 |
| MDL Number | MFCD00133991 |
| Solubility | Soluble in water |
| PH | 7.0 - 7.6 at 25°C |
Protocol
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To prepare a solution of 4% paraformaldehyde you need to use this protocol. The following ingredients are required:
•1M Sodium Hydroxide
•10X phosphate-buffered saline (PBS)
•1M Hydrochloric acid
Note: This should be done in a fume chamber with gloves, safety glasses, and a face mask.
1. To prepare a 4% of paraformaldehyde (PFA) solution in PBS, add 4 grams of granular or prill paraformaldehyde to water (H2O)
2. Use stirring hot plate to heat the solution to 60-degree celsius. Add 1mL of 1M NaOH until the paraformaldehyde is fully dissolved.
3. Add 10 mL of 10x phosphate-buffered saline (PBS) and allow the mixture to cool.
4. Adjust the solution pH with 1M HCL then top up the volume to 100 mL with H2O.
5. Filter the solution to remove any deposit.