The 14-3-3 zeta Colorimetric Cell-Based ELISA Kit provides a lysate-free, convenient, and high-throughput approach for sensitive detection of 14-3-3zeta protein expression profile in cells. The kit can be used for measuring the relative amounts of 14-3-3zeta in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on 14-3-3zeta.